Impact of high protein diet on the formation and healing of L-arginine induced acute pancreatitis in male wistar rats

Tosan Peter Omayone; Ibrahim Aliyu; Grace Iyabo Adebayo-Gege

doi:10.46481/asr.2025.4.1.187

Authors

Tosan Peter Omayone Department of Physiology, School of Basic Medical Sciences, College of Health Sciences, Federal University of Technology, Akure, Ondo state, Akure, Nigeria
Ibrahim Aliyu Department of Physiology, School of Basic Medical Sciences, College of Health Sciences, Federal University of Technology, Akure, Ondo state, Akure, Nigeria
Grace Iyabo Adebayo-Gege
[email protected]
Department of Physiology, Faculty of Basic Medical Sciences, College of Medicine and Health Sciences, Baze University, Abuja, Nigeria

Keywords:

High protein diet, L-arginine, Acute pancreatitis, Antioxidants, MDA

Abstract

The condition known as acute pancreatitis is defined as the inflammation of the pancreas, commonly caused by gallstones and alcohol ingestion. The paucity of information on its management in combination with other factors such as adverse effects resulting from treatment has redirected the attention of researchers to safer, alternate therapies. This study aims to assess the role of a high-protein diet in mitigating acute pancreatitis caused by L-arginine. Two groups of twenty male rats were randomly assigned; groups fed with normal diet (NP), and groups fed with high-protein diet (HPD). Acute pancreatitis was induced with L-arginine monohydrochloride at dose of 250mg/Kg. It was administered 3 times at interval of one hour. After induction, the groups were further grouped into subgroups upon observations on day 3 and day 7. Lipid peroxidation (MDA level), total protein and antioxidants parameters such as hydrogen peroxide, nitric oxide. sulfurhydric acid concentration, total antioxidant capacity (TAC), CAT, GPx and NO were evaluated using spectrophotometry. Every data set was shown as mean °æ SEM and as an ANOVA with a post-hoc analysis at α= 0.05. Findings revealed that the high protein diet administered significantly increased the protein level, sulfurhydryl concentration, TAC, CAT, GPx and NO compared to the acute pancreatitis model on day 3, and had no significant effect in most parameters on day 7. Lipid peroxidation substantially decreased in the treatment groups when compared to pancreatitis model. High protein diet improves the amelioration of acute pancreatitis by enhancing the antioxidant enzymes, nitric oxide and inhibiting the process of lipid peroxidation.

Dimensions

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